FastQCFastQC Report
Mon 6 Jun 2016
ERR1390703.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390703.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858739
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC507.172229E-526.99119644
CTCGTAT602.472255E-422.49266442
CCGGCTA1204.5292836E-1022.49266416
TCGTATG602.472255E-422.49266443
CGGCTAA1204.5292836E-1022.49266417
CGTTCAT706.996838E-419.27942732
ACCGTTC706.996838E-419.27942730
ATCGGAA1051.449218E-619.27942719
AGCGCTC851.2037753E-418.5298427
AGATCGG1102.2616678E-618.40308817
GATCGGA1102.2616678E-618.40308818
CGCCCGG1102.2616678E-618.40308813
ACGCCCG750.001112343617.99413312
TCTCGTA750.001112343617.99413341
CCGTTCA750.001112343617.99413331
GCCCGGC1902.3646862E-1117.75736614
TCGGAAG1053.1196592E-517.13726820
AAAGCGC1205.069427E-616.9098665
CGGTTCA1205.1861043E-616.86949729
GCGGTTC1104.648281E-516.35830128
Produced by FastQC (version 0.11.5)