FastQCFastQC Report
Mon 6 Jun 2016
ERR1390695.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390695.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894431
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG403.4648292E-739.36374732
CCGTTAC458.7165245E-734.98999831
TATCTCG501.9845247E-631.49276239
ACCGTTA608.207082E-626.242530
GATCGGA651.5253134E-524.22655518
CGTATGC651.5266349E-524.22384644
GACCGTT651.5266349E-524.22384629
CACCGCG500.00226503722.4986028
ATCGGAA702.705761E-522.49608619
ACGTATC702.70693E-522.49482736
GTTACGT702.7080994E-522.4935733
TCTCGTA702.7080994E-522.4935741
TCGTATG855.0426133E-621.17042243
AGATCGG754.6073368E-520.99634717
GAGACCG908.264289E-619.99540327
TACGTAT908.264289E-619.99540335
ATCTCGT807.573972E-519.68297440
CGTATCT807.573972E-519.68297437
AGACCGT807.573972E-519.68297428
CTCGTAT851.20670215E-418.52411842
Produced by FastQC (version 0.11.5)