FastQCFastQC Report
Mon 6 Jun 2016
ERR1390688.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390688.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527217
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC5840.1107703279674214Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATT453.4509678E-530.0460471
AGCGACT300.005151552629.99190731
CCGAGAC1300.025.95453645
TCGGAAG1159.094947E-1225.42792320
GATCGGA1201.6370905E-1124.36842518
AGATCGG1252.910383E-1123.39368617
CGGAAGA1305.0931703E-1122.4939321
ATCGGAA1305.0931703E-1122.4939319
GCGGTTC1459.094947E-1221.71827528
CGGTTCA1552.3646862E-1120.31709929
AGCGGTT1703.6379788E-1219.84758427
GAGCGGT1603.8198777E-1119.6821926
CCGAGAT1603.8198777E-1119.6821914
AGAGCGG1603.8198777E-1119.6821925
CAAACCG600.00644149218.782355
AAGAGCG1709.094947E-1118.52441224
GAGATCG1608.1490725E-1018.27631816
CGCCACC1002.0131341E-518.0310555
ACGCCCA750.001111149817.99514412
CGAGATC1904.474714E-1016.57447615
Produced by FastQC (version 0.11.5)