FastQCFastQC Report
Mon 6 Jun 2016
ERR1390686.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390686.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521105
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC6090.11686704215081414Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC458.7204717E-734.98320844
CTCGTAT458.7204717E-734.98320842
CGATTAC501.9862946E-631.48488832
CCGATTA608.210853E-626.23740831
GTATGCC608.210853E-626.23740845
TCGTATG608.210853E-626.23740843
TCTCGTA651.5273401E-524.21914541
CATCTCG702.705341E-522.49352339
GACCGAT702.7093558E-522.48920629
AGACCGA702.7093558E-522.48920628
ACGGGAT855.0402286E-621.16834313
ACCGATT754.6134628E-520.98992330
CTTCGTA654.231201E-420.7712257
AGATCGG654.2449305E-420.7612617
GATCGGA550.003942451420.44669313
GAACCCG550.003944643320.44473321
ATCTCGT807.569602E-519.68183140
CGTTGGA1051.4057423E-619.3376925
CCTTCGT706.877874E-419.326531
AACCCGG600.006523877418.74100522
Produced by FastQC (version 0.11.5)