FastQCFastQC Report
Mon 6 Jun 2016
ERR1390685.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390685.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462675
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT453.4919904E-529.99293942
CGTATGC507.160777E-526.99364544
CGTTGAC507.160777E-526.99364532
CCGTTGA551.3686623E-424.53967931
GACTATC551.3686623E-424.53967936
ATCTCGT602.4683485E-422.49470540
TTCCCGT602.4683485E-422.49470523
TCTCGTA602.4683485E-422.49470541
TCGTATG602.4683485E-422.49470543
CCCGGCC754.6033456E-520.99505615
GTATGCC654.2396868E-420.76434345
GATCGCA550.003904007420.480724
AGATCGG1009.051364E-720.24523417
GATCGGA908.250532E-619.99529318
TATCTCG706.985897E-419.28117639
CCGGGTT600.00650547518.749649
AGACCGT750.001110613817.99576428
ATCGGAA1002.046483E-517.99576419
CCGAGAC1153.4473887E-617.60455145
TCGGAAG1053.1122112E-517.1388220
Produced by FastQC (version 0.11.5)