FastQCFastQC Report
Mon 6 Jun 2016
ERR1390683.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390683.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606298
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT751.7029342E-623.99256129
GATCGGA802.9823823E-622.49442918
CGTTAAC802.983994E-622.49302732
TCGCTGC550.003944282420.44820831
GCGTGCG550.003944993520.4475712
ACCGTTA908.276746E-619.99380130
AGATCGG951.3191779E-518.94267517
AACGGGT600.006524429718.74360712
AGACCGT1002.0523748E-517.99498228
CCGTTAA1053.1219613E-517.13754531
GCCCGCT800.001714440116.87029528
ATCTCGT1205.19097E-616.86977240
CGGGATT1904.5110937E-1016.5748414
TCTCGTA952.8269054E-416.57380941
ATCGGAA1104.649209E-516.35958519
CTCGTAT1004.178166E-415.74511942
TCGGAAG1156.803173E-515.64732320
ACGGGAT2253.0559022E-1014.99581713
ACGAAAT2001.5797923E-814.6213789
TCGTATG1251.3850031E-414.39508943
Produced by FastQC (version 0.11.5)