FastQCFastQC Report
Mon 6 Jun 2016
ERR1390682.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390682.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837596
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACG206.962535E-445.091012
AGATCGG453.4971286E-529.99068617
ATCGGAA406.17594E-428.1162719
GATCGGA450.001228498424.99223918
ACCGCGC654.2457934E-420.7627859
TCGGAAG550.003942742520.44819620
CACCGCG706.995904E-419.2797288
CCGCGCC850.00256970315.87742210
CGAGGCA1156.796993E-515.64731433
GATCTCG1402.2350761E-514.45979618
TCTTCGT950.005302841314.2392676
CAACGAA1301.9302222E-413.8418577
ATCTCGG2154.0812665E-813.60042819
CGTGAGC1000.007448544713.5273031
CCAACGA1403.543642E-412.8831466
TCCAACG1454.7642825E-412.4389015
ACCACGC1651.0452724E-412.2689189
CCACGCC1651.0452724E-412.26891810
TCGGCTC2054.8381316E-612.06942422
GGCCGAG2158.225803E-611.50805543
Produced by FastQC (version 0.11.5)