FastQCFastQC Report
Mon 6 Jun 2016
ERR1390678.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390678.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931227
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC16050.1723532500668473Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCTA250.002107520835.99785210
CGCATTA1450.032.57551632
TCGTATG1450.032.57551643
CGACATA352.8254034E-432.1323121
ACCGCAT1550.030.4738730
GACCGCA1550.030.4738729
TCTCGTA1550.030.4738741
CGGTGAA300.005155027429.99015825
AGACCGC1600.029.52155928
CGTATGC1700.027.78499644
CCGCATT1700.027.78499631
GATCGGA3050.027.28612518
CTCGTAT1650.027.26377942
AGATCGG3100.026.84602717
ATCGGAA3200.026.70998219
TCGGAAG3300.025.21899620
ATCTCGT1800.024.99179640
CATCTCG1900.024.86026239
GAGACCG1950.024.22281827
CCGAGAC3600.023.11741345
Produced by FastQC (version 0.11.5)