FastQCFastQC Report
Mon 6 Jun 2016
ERR1390676.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390676.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839238
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG501.9862073E-631.48894743
ACCGCTC406.175259E-428.11680830
CCGCTCT450.001228363424.99271831
CGCTCTA450.001228363424.99271832
CGTATGC450.001228790124.99122844
AGATCGG751.7010425E-623.99300817
GATCGGA802.980696E-622.49344618
AGACCGC602.4716073E-422.49344428
GGCGGTG500.002267940722.49344431
CTCGTAT500.002268726222.49210442
CGGGATG500.002268726222.49210423
ATCGGAA855.042246E-621.17030119
CCGAGAC855.044849E-621.1690445
GACCGCT550.003942311720.44858729
GAGACCG550.003942311720.44858727
TCGGAAG1009.0794674E-720.24289320
CCGCGCC600.006439686318.7848323
TCTCGTA600.00652226518.74342241
CCGGCTA1053.118593E-517.13786316
CGGCTAA1209.7713935E-514.9956317
Produced by FastQC (version 0.11.5)