FastQCFastQC Report
Mon 6 Jun 2016
ERR1390675.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390675.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879535
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGGT453.497876E-529.98999627
CTTCCGG706.9973763E-419.27928226
CCGCGCC600.006506885418.75120710
GCCACCG1301.0850312E-515.6081086
TCGTTGG1951.1010343E-815.0248944
TTCGTTG2001.5386831E-814.6484373
ACGGGAT1251.3831194E-414.39601613
CTCGCTC950.00531387414.234921
CTCGTGA1453.1124884E-513.96085942
CCACCGC1150.001200800113.6965357
CGTTGGA2205.3525582E-813.3220735
ATCTCGG1902.0661482E-613.02271219
CGTGAGC1300.002981963312.1361821
CTCGGCT2054.84016E-612.06914421
CGGGATT1506.4613245E-411.9966814
TACTAGG1350.00401579911.66277621
AAACGGG1953.840797E-511.53854911
TCGGCTC2158.22923E-611.50778922
TCTCGGC2158.22923E-611.50778920
AACGGGA1802.3501799E-411.24624812
Produced by FastQC (version 0.11.5)