FastQCFastQC Report
Mon 6 Jun 2016
ERR1390673.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390673.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495383
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCCGG300.005150845329.99239313
GATCGGA406.17029E-428.11787218
ACCCGGC450.001227381424.99366414
TATCTCG500.002266140722.49429539
AGATCGG600.00651491618.74524517
ATCGGAA600.00651491618.74524519
TCGGAAG600.006518714618.74335520
CGTTGGA901.833692E-417.536285
TCGTTGG1053.051891E-517.1783964
GGTTGCA2101.8480932E-914.99619837
GATACTA1108.599234E-414.313107521
CCCCTAC950.005382944414.2054910
CCCTACA1150.001203303213.69079811
ATACTAC1150.001203303213.69079822
GTTGCAG2205.4937118E-813.29208438
TATCTTC5150.013.1339684
TACAAGC1200.001657252713.12167214
GAAGCAT4600.012.22392727
ATATCTT5350.012.2215463
AGGTTGC2054.8238326E-612.0701136
Produced by FastQC (version 0.11.5)