FastQCFastQC Report
Mon 6 Jun 2016
ERR1390669.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390669.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869684
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCTA458.721854E-734.9870831
CGCCTAA501.986602E-631.48836732
CGTCTTG300.0051558829.98892232
AGATCGG1200.026.24332217
GATCGGA1251.8189894E-1225.19359218
ATCGGAA1251.8189894E-1225.19359219
TCGGAAG1353.6379788E-1223.32471720
GCGGTTC1450.023.26726528
GACCGCC702.7097347E-522.49169329
AGACCGC702.7097347E-522.49169328
CGGTTCA1551.8189894E-1221.76615329
ACCGCCT754.61409E-520.99224730
TCGTATG754.61409E-520.99224743
AACGTCC550.003938955320.4516968
GAGCGGT1653.6379788E-1220.44699326
CGGAAGA1552.3646862E-1120.31507721
CGAGATC1703.6379788E-1219.84789515
CCGAGAC1703.6379788E-1219.84561245
CTCGTAT807.581786E-519.6802342
TCTCGTA807.581786E-519.6802341
Produced by FastQC (version 0.11.5)