FastQCFastQC Report
Mon 6 Jun 2016
ERR1390667.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390667.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812008
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG1204.5292836E-1022.49408517
GATCGGA1204.5292836E-1022.49408518
ATCGGAA1301.2296368E-920.76377519
CGGAAGA1301.2314558E-920.76249521
CCGAGAC1403.09592E-919.2794645
TCGGAAG1507.2850526E-917.99416420
AGCGGTT1703.4120603E-815.87817927
CCGAGAT1703.4120603E-815.87817914
AAGAGCG1754.8763468E-815.42356824
GCGGTTC1859.6306394E-814.5907628
ATCTTCG950.005309249314.23666
GAGCGGT1951.8338324E-713.84166326
CGGTTCA2002.4949077E-713.49645329
GAGATCG2053.3693686E-713.16727216
ATGCCGA2309.838186E-812.71561511
CGAGATC2207.9341044E-712.26950215
AATGCCG2401.7129969E-712.18579910
AGAGCGG2251.0418044E-611.99610925
TCTCGAA1953.7690108E-511.5596325
CTCGAAC1600.001090426611.2706416
Produced by FastQC (version 0.11.5)