FastQCFastQC Report
Mon 6 Jun 2016
ERR1390665.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390665.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981998
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG300.005150486729.9957247
TGACGAA300.005154335429.9911431
GATCGGA608.205516E-626.24358418
ATCGGAA608.205516E-626.24358419
AGATCGG651.5263438E-524.22484817
TCGGAAG651.5269461E-524.22361420
GCGGTTC908.270323E-619.99409328
CGGAAGA901.8695454E-417.49483121
CCGAGAC1053.1195745E-517.13779445
GAGCGGT1104.6481502E-516.35880326
CTTCGTA850.002567248715.8800897
CTCGGGA1901.3359386E-714.20632935
CTACTCG1301.930509E-413.84206532
AGCGGTT1352.6579664E-413.32939427
CGGTTCA1200.001659795213.12112329
TCGTATA1250.00225352512.5982039
TGCCGAG1558.511462E-411.60947312
ACCACGC1802.346549E-411.2483969
TACTCGG1600.001110380711.24667733
AGAGCGG1400.00526680211.24667725
Produced by FastQC (version 0.11.5)