FastQCFastQC Report
Mon 6 Jun 2016
ERR1390664.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390664.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720321
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG458.719471E-734.9868820
AGATCGG501.9849886E-631.49037717
GATCGGA501.9849886E-631.49037718
AACCGTA300.005153689529.99083932
ATCGGAA602.4233304E-729.99083919
GAGCGCT500.002265988822.496258
GCGGTTC802.9800885E-622.49312828
GAGCGGT903.2952994E-722.49156626
CGGAAGA754.6128764E-520.99212821
AGCGGTT908.266023E-619.9938927
TCGTTGG1151.5613841E-719.6027894
CGTTGGA1051.4149045E-619.3267825
AGCGCTT600.00651445418.7468769
AGAGCGG1053.119837E-517.13643325
CGGTTCA1104.645787E-516.35863729
GAGATCG1004.174038E-415.74518916
TTCGTTG1351.5504844E-515.0298493
ATGCCGA1209.757957E-514.997511
AATGCCG1402.2355778E-514.45886241
CTACTCG1150.001203787413.6914732
Produced by FastQC (version 0.11.5)