FastQCFastQC Report
Mon 6 Jun 2016
ERR1390663.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390663.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920615
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA702.8927388E-828.92043318
ATCGGAA801.056178E-725.30538219
TCGGAAG851.8979517E-723.8168320
AGATCGG903.29439E-722.49367117
ACGCCCG602.4726012E-422.4924512
AGACCGT600.006518069718.74574528
CCGTAAG600.00652011418.74472631
ACCGTAA600.00652011418.74472630
AAAGCGC1205.0810922E-616.9060675
GCGCAAT800.001714340316.86933914
CCGGCTA1501.4272518E-716.49446316
GCGGTTC1104.644919E-516.35992228
CGGCTAA1703.414607E-815.87788617
CGCTTTG850.002570393515.87702345
TCGTTGG2051.2860255E-915.3907914
CTTCGTT1502.527724E-615.0251612
AGCGGTT1209.767674E-514.99659527
AGAGCGG1209.767674E-514.99659525
ACCTCCG1056.048136E-414.99578235
CTCGTAT900.003761553214.994966542
Produced by FastQC (version 0.11.5)