FastQCFastQC Report
Mon 6 Jun 2016
ERR1390659.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390659.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138320
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC12250.10761473048000562Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGA300.00515631229.98919712
AGATCGG2300.028.35934817
GATCGGA2450.027.54109818
ATCGGAA2700.025.82402819
TCGCACA450.001229125824.99099720
TCGGAAG2850.024.46486920
ATCTCGT1353.6379788E-1223.32492840
CGTATGC1305.0931703E-1122.49189644
CTCGTAT1401.382432E-1020.88533242
CGAGAAC550.003943732520.44807637
TATCTCG1503.45608E-1019.49383439
CCGAGAC3400.019.18426545
CGGATCA600.006524011518.74324811
GGCGGAT600.006524011518.7432489
GCGGATC600.006524011518.74324810
GACCGTT1709.276846E-1118.52355229
TCTCGTA1608.203642E-1018.27466641
TCGTATG1608.203642E-1018.27466643
CGAGATC3600.018.11847315
AGCGCTC1002.0529584E-517.9935177
Produced by FastQC (version 0.11.5)