FastQCFastQC Report
Mon 6 Jun 2016
ERR1390658.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390658.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865429
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACCG250.00211019735.9882629
CGTTTAG401.5636107E-533.73899532
TATGCCG300.00515477729.9902231
GACGAAA450.001228643924.99184639
CGTGCAC500.002238159322.5447645
GATCGGA702.7076027E-522.49396318
GAGACCG602.4722764E-422.49266427
CGTCTGG500.002268456622.49266234
CCGTTTA802.9817784E-622.49266231
GACCGTT654.2464002E-420.76245929
TCGTATG654.2464002E-420.76245943
AGCACGG550.003941886620.44905514
CCGTCTG550.003943206320.44787433
AGATCGG807.575854E-519.68221517
ACCGTTT807.579219E-519.6810830
AGACCGT706.996899E-419.27942528
GAGCGTG600.00642635218.7916561
ATCTCGT851.2070191E-418.52336940
CGTATGC750.001112353417.99413144
CTCGTAT750.001112353417.99413142
Produced by FastQC (version 0.11.5)