FastQCFastQC Report
Mon 6 Jun 2016
ERR1390657.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390657.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232716
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG300.005155189429.99072529
GCTACGG300.005155189429.99072525
CGTATGC608.211866E-626.24188444
TCGGAAG1700.023.81616420
ATCGGAA1700.023.81616419
GATCGGA1750.023.13570218
GTTTACG802.9819912E-622.49395833
CCGAGAC1950.021.91629845
AGATCGG1850.021.88512217
GACCGTT855.046195E-621.16992429
TCTCGTA754.6139612E-520.99350741
TACGATC807.579198E-519.68221336
CTCGTAT807.581561E-519.68141442
ACGATCT1051.4494672E-619.28053337
AGACCGT951.3194709E-518.94151128
CGGTTCA2200.018.40339929
GAGACCG1053.1209907E-517.13755627
CCGTTTA1053.1209907E-517.13755631
GCGGTTC2400.016.86978128
ATCTCGT1104.6502493E-516.35857640
Produced by FastQC (version 0.11.5)