FastQCFastQC Report
Mon 6 Jun 2016
ERR1390655.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390655.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664589
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTCG250.002109152335.99066538
GATCGGA1058.54925E-1125.70762318
GTAGGGC551.3690625E-424.5409415
AGATCGG1152.6557245E-1023.47394217
CGAGATC1204.5110937E-1022.49586115
GAGATCG1358.367351E-1121.6626816
TCGGAAG1301.2296368E-920.76228520
ATCGGAA1301.2296368E-920.76228519
CACTCGT550.003896278820.4893285
CCGAGAC1351.9681465E-919.9933145
CCGAGAT1403.086825E-919.28216714
GACCGTT600.006517419618.74513829
CGTATGC600.006520251318.74372944
GAGCGGT1507.2723196E-917.99533526
GCGGTTC1601.6148988E-816.87062628
CTTCGTT1104.5629E-516.3926982
AAGAGCG1652.3603207E-816.35939424
AGCGGTT1652.3603207E-816.35939427
CGGAAGA1652.3625034E-816.35816421
CCACTCG850.002534734315.9093624
Produced by FastQC (version 0.11.5)