FastQCFastQC Report
Mon 6 Jun 2016
ERR1390653.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390653.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090481
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGG250.002109781635.99053629
TAGCGTG300.00515260929.99348816
GTAGCGT300.00515260929.99348815
GCGCGGC300.005153764529.99211530
GCGTATC300.005153764529.99211533
GATCGGA709.328178E-725.70752718
CTCGTAT551.370583E-424.53900342
CCATCGT551.370583E-424.53900342
TCGTATG551.370583E-424.53900343
GACCGTT754.611689E-520.99447829
GCGAAAC654.1893855E-420.804791
CCGCCAC550.003900709620.4867444
ATCTCGT706.9955195E-419.28064540
ATCGGAA951.31871675E-518.94238919
AGATCGG851.206375E-418.52538917
CGCCACC1354.195317E-818.3621925
TCTCGTA750.001112134217.99526841
AGACCGT750.001112134217.99526828
CGTATGC800.001713780916.87056444
CGAAATC1104.645789E-516.36008510
Produced by FastQC (version 0.11.5)