FastQCFastQC Report
Mon 6 Jun 2016
ERR1390651.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390651.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833421
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGGT300.0051021430.0531022
TGATCGG300.005153339429.991826
AAACGAC300.005153339429.991837
TACGGAA406.105559E-428.1713963
ATGCCGT507.169404E-526.9926244
CGGGCGT450.001212841625.0472641
TATGCCG450.001228227424.99316843
AGATCGG1201.6370905E-1124.36979917
CCGTGTA851.8973515E-723.81701931
GATCGGA1252.910383E-1123.39360418
ATCGGAA1353.6379788E-1223.32695819
GACCGTG903.293353E-722.4938529
GAGACCG955.542515E-721.30996327
GCTACAC550.003941879620.44895619
CGTATGC1009.072555E-720.24446544
AGACCGT1009.072555E-720.24446528
TCGTATG1051.4483812E-619.28044543
TCGGAAG1656.002665E-1119.0856920
CCGAGAC1656.002665E-1119.0856945
CCGGCTA600.00651930918.74487528
Produced by FastQC (version 0.11.5)