FastQCFastQC Report
Mon 6 Jun 2016
ERR1390650.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390650.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536184
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCCG352.7779545E-432.2220884
ACCGATC352.8240107E-432.1319330
CTCGTAT352.8255567E-432.12893742
CGCATAT300.005155747329.98700541
CGCTAAT300.005155747329.98700522
ATCTCGT450.001228800424.98917240
GACCGAT500.002267520222.49235329
AGATCGG802.9786243E-622.49235217
CGTATGC500.002268749522.49025544
TCTCGTA500.002268749522.49025541
TCGTATG500.002268749522.49025543
TCGGAAG855.0428334E-621.167320
CCGATCA550.00394372420.44568431
CGATCAA550.00394372420.44568432
ATCGGAA908.268673E-619.99133719
GATCGGA951.3186103E-518.93916118
AGACCGA600.006518851518.74362828
CGGGGTT1002.0004893E-518.0443693
CCGAGAC1053.1189935E-517.13543145
ACTAGAT952.825157E-416.57176622
Produced by FastQC (version 0.11.5)