FastQCFastQC Report
Mon 6 Jun 2016
ERR1390649.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390649.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246391
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGT300.00515537429.99053431
GATCGGA1052.3756002E-923.56493618
CACTCGC602.472277E-422.49380336
AGATCGG1351.9717845E-919.9944917
TCGGAAG1351.9717845E-919.9944920
ATCGGAA1251.7733328E-819.79454619
ACGCCCG807.5820215E-519.68128812
CGGTGGA952.8255244E-416.5743814
GCGGTTC1552.0719744E-715.963984528
CTTCGTA1552.0738662E-715.9627037
TCGTATA1209.779477E-514.9952679
CGTATAA1502.582381E-614.99526610
ATGCCGA1806.899427E-814.99526611
GAGCGGT1705.904476E-714.55539726
CGGAAGA1705.909824E-714.5542321
TGTCGCC1251.3843097E-414.3954558
GTTTCGC950.005312077214.2363123
CCGAGAC1758.199295E-714.13839445
TCGTTGG2254.773028E-914.0254034
CACGCCC2154.087815E-813.60035711
Produced by FastQC (version 0.11.5)