FastQCFastQC Report
Mon 6 Jun 2016
ERR1390648.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390648.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231709
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAC250.00210950135.9918829
GACCGGT654.8852235E-727.68493829
ACCGGTA709.330088E-725.70744330
CCGGTAA855.044307E-621.17083531
CGGTAAA855.044307E-621.17083532
GATCGGA754.6080993E-520.99696718
TGTCCCG550.00393998720.45159319
CGTATGC908.271038E-619.99467844
CTCGTAT908.271038E-619.99467842
TGTAACG600.00652076618.7450145
ATCGGAA1053.1165728E-517.14038319
TCTCGTA1053.1198324E-517.13829641
AGATCGG952.8226673E-416.57655317
TATCTCG1104.648528E-516.35928239
TCGTATG1104.648528E-516.35928243
CGTGAGC1502.5366899E-615.0210091
CGCCACC1502.5366899E-615.0210095
CGAAATC1351.5851114E-514.99661710
ACCACGC1859.640098E-814.5913049
AGACCGG1301.9286649E-413.84415528
Produced by FastQC (version 0.11.5)