FastQCFastQC Report
Mon 6 Jun 2016
ERR1390647.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390647.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662397
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC8810.13300181009273893Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT504.885078E-835.990442
CGTATGC708.1490725E-1032.13428544
ACGCCAC300.00515051629.99426326
TATCTCG602.42042E-729.99426339
TCGTATG803.3996912E-928.11749843
TCTCGTA654.8794936E-727.68492341
AGACCGT709.31308E-725.70936628
CCGTGAA709.3192284E-725.70742631
CCGAGAC1700.025.1403545
ATCTCGT751.6997765E-623.99359940
GATCGGA1850.023.10368718
GACCGTG802.976536E-622.49569729
ATCGGAA1900.022.49419
CGTGAAA855.0385243E-621.17082232
ACCGTGA855.0385243E-621.17082230
TCGGAAG2050.020.84809720
GTATGCC1109.996256E-820.4490945
AGATCGG2100.020.3532517
GGCGGGT706.991735E-419.2805719
GAGGTCG600.006514839418.74641427
Produced by FastQC (version 0.11.5)