FastQCFastQC Report
Mon 6 Jun 2016
ERR1390646.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390646.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648829
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAC300.00515472429.99187336
ACCGCGC952.3646862E-1128.412499
CGCGCCC856.504706E-926.46341711
CACCGCG1058.54925E-1125.7065418
GTACGAA551.3710737E-424.53880534
CGCCCGG1600.023.90122213
AATCCGT500.002268592822.49390435
CGTATGC702.7102133E-522.49322344
ACGCCCG1358.367351E-1121.66079712
CCGCGCC1358.367351E-1121.6601410
GACCGTA754.6138128E-520.99431229
TAGTACG654.2479348E-420.76297632
AGTACGA706.997978E-419.2804933
ACCGTAG951.3194769E-518.94223630
CCGGCTA2500.018.89602516
CGGCTAA2500.018.89602517
CCGAACT600.00652186118.7449233
CTGGCGA600.00652186118.7449238
GGTTTCG1205.0926574E-616.903754
GCGCCCG800.001714375816.87042812
Produced by FastQC (version 0.11.5)