FastQCFastQC Report
Mon 6 Jun 2016
ERR1390643.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390643.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1370932
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGT709.335763E-725.70605532
CTCGTAT709.335763E-725.70605542
AGATCGG1101.5279511E-1024.53759617
CCCGCCA1400.024.1558113
GACCGGA802.9837302E-622.49279829
CCGCCAC1750.021.9012684
TCTCGTA855.0473554E-621.16969141
CGCCACC1850.020.7174175
AACGAGT550.003891197720.4958384
CTATCGC550.00394339420.44874238
GATCGGA1251.774606E-819.79366318
TCGGAAG1251.774606E-819.79366320
ATCTCGT1051.450424E-619.27954140
ATCGGAA1403.101377E-919.27954119
CGAGTTT600.00643303418.7892271
GTCGGGA600.00652179618.7446835
GGTGCGA600.00652316918.74399813
CGCCCGC901.8364217E-417.5366121
ACGCCCG901.8705823E-417.49439812
ACCGGAA1053.1216958E-517.1373730
Produced by FastQC (version 0.11.5)