FastQCFastQC Report
Mon 6 Jun 2016
ERR1390641.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390641.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533336
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1700.031.75540518
AGATCGG2050.026.3354717
TTGGCGC450.001228370524.9943114
ATCGGAA2150.024.06193419
GCGGTTC2350.022.97199628
ATCCGAG500.002268379322.49414634
GAGCGGT2400.022.49341226
CGGTTCA2550.022.05236629
CCGAGAC2450.022.03292745
TCGGAAG2350.022.01339120
AGCGGTT2550.021.1702727
TCGCGCC754.536051E-521.040516
GATCGCG654.184339E-420.8092964
CGAGATC2650.020.3727215
TCGCACC706.894915E-419.3229166
ATCGCGC706.894915E-419.3229165
AGAGCGG2800.019.28006725
ACCACGC2150.018.8304659
ATTCGCC600.006439415718.7861716
CCGTAGA600.00652493618.7432931
Produced by FastQC (version 0.11.5)