FastQCFastQC Report
Mon 6 Jun 2016
ERR1390640.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390640.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683245
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCCTCATCTCGTATGCC13820.2022700495429897Illumina Paired End PCR Primer 2 (97% over 36bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7200.10537947588346787Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1500.043.4887543
TCTCGTA1750.039.84683641
CATCTCG1650.039.5352339
CGTATGC1700.038.3724344
CTCGTAT1700.038.3724342
ATCTCGT1750.037.27607340
CGTTCCT1750.037.27607332
ACCGTTC1750.037.27607330
GACCGTT1850.035.2611529
AGACCGT1950.033.45288528
GAGACCG2100.031.06339527
TCGATGG300.00510822230.0450175
GTATGCC2200.029.6514245
CCGTTCC2250.028.992531
CCGAGAC2500.026.09325225
AGATCGG1550.024.67284417
CGAGACC2800.024.10090826
TCGGAAG1600.023.90006620
GATCGGA1650.023.17751918
CCGGCTA1003.6459824E-822.49582916
Produced by FastQC (version 0.11.5)