FastQCFastQC Report
Mon 6 Jun 2016
ERR1390638.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390638.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856458
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGC250.002108728535.9933211
TAAGACG250.002108728535.9933210
TTCGCAC250.002109937635.98911725
GATGCGT406.175761E-428.11649916
ATGCGTT406.175761E-428.11649917
ACCGCGC450.001227626624.9953619
CGCGCCC450.001227626624.99536111
CCGCGCC450.001227626624.99536110
ACCCGGC651.5267324E-524.22344414
CACCCGG651.5267324E-524.22344413
AGATCGG1052.3737812E-923.56430217
GATCGGA1052.3737812E-923.56430218
CGAGTTG500.00226812322.49319832
ATCGGAA1104.0636223E-922.49319819
AGATGCG500.00226812322.49319815
CCGAGAC1156.7866495E-921.51523445
GCGCCCG550.003939962520.4507512
CCACCGC908.260453E-619.9962887
CCCGGCC807.577712E-519.6815515
TCGGAAG1302.7794158E-819.03270520
Produced by FastQC (version 0.11.5)