FastQCFastQC Report
Mon 6 Jun 2016
ERR1390636.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390636.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720071
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC10530.14623557954701688Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA207.0421235E-444.98624816
TCGACAC207.0421235E-444.98624817
CGTATGC803.6379788E-1233.73734344
TCTCGTA855.456968E-1231.75279641
CCGGTAT855.456968E-1231.75279631
CTCGTAT901.2732926E-1129.98875242
GATCGGA2200.028.62761318
AGATCGG2250.027.99144417
ATCTCGT1053.6379788E-1227.84669740
GACCGGT1004.5474735E-1126.9917529
AGACCGG1004.5474735E-1126.9917528
ACCGGTA1004.5474735E-1126.98987630
TCGTATG1004.5474735E-1126.98987643
CGGTATG1004.5474735E-1126.98987632
CCGAGAC2400.026.24015645
ATCGGAA2400.026.24015619
TCGGAAG2450.025.70464120
GAGACCG1152.6557245E-1023.47108827
GGATCTC1305.0931703E-1122.49468838
CCGAGAT2800.022.49312414
Produced by FastQC (version 0.11.5)