FastQCFastQC Report
Mon 6 Jun 2016
ERR1390634.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390634.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395974
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGT803.6379788E-1233.7388529
CGTATGC855.456968E-1231.75421344
TCGTATG855.456968E-1231.75421343
CTCGTAT901.2732926E-1129.9900942
CCGGTTG901.2732926E-1129.9900931
ACCGGTT952.3646862E-1128.41166530
TTTAGCG406.17874E-428.1157141
AGACCGG1004.5474735E-1126.99204628
CGGTTGG1004.5474735E-1126.99108132
TCTCGTA1159.094947E-1225.4263841
ATCTCGT1101.5279511E-1024.53734640
GCGAAAG500.002238675322.5450555
ATTCCGC500.002269203822.49256712
GAGACCG1358.367351E-1121.66028627
GATCGGA1555.366019E-1018.86541218
AGATCGG1801.0913936E-1118.74447817
CTCGTTA600.006522260618.74447614
CAAATCG600.006522260618.74447614
AGGCCGC600.006523609618.74380734
ATCGGAA1651.2350938E-917.72205219
Produced by FastQC (version 0.11.5)