FastQCFastQC Report
Mon 6 Jun 2016
ERR1390633.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390633.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135513
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGGT250.002110014435.98985313
TTAGCGT300.00515433129.99154513
TACATCG300.005155441429.9902258
CCGCAAG554.1754593E-628.62703131
AGCTACG507.17207E-526.99239235
CGCAAGG608.212186E-626.24144732
CTCGTAT651.5275802E-524.22287442
TATCTCG702.7088478E-522.49365839
TCTCGTA702.7097694E-522.49266841
ATCGGAA1257.548806E-1021.5929619
AGACCGC754.6094465E-520.99593228
ACCGCAA754.611011E-520.99500730
CGTATGC754.6141417E-520.99315844
ACGCCCG550.003942941320.4487812
TGTCGAG550.00394394720.447888
GATCGGA1351.9717845E-919.99436218
GACCGCA807.574164E-519.68368529
TCGTATA1051.4500547E-619.279439
TGTGCGG600.006521051318.74471733
AGATCGG1454.789399E-918.6154417
Produced by FastQC (version 0.11.5)