FastQCFastQC Report
Mon 6 Jun 2016
ERR1390625.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390625.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600739
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCG850.034.4010130
CGGCGTA950.033.14753332
CTCGTAT950.033.14753342
TCTCGTA950.033.14753341
TCGTATG950.033.14753343
ATCTCGT1000.031.49015440
CGTATGC1000.031.49015444
GACCGGC950.030.77985229
TGAACCG300.005094730330.0606823
ACTCGCA300.005153196429.99062319
CTCGCAA300.005153196429.99062320
CGTGTAA300.005153196429.99062342
CGGAGGG300.005153196429.99062317
GGCGTAA1150.029.33865233
CCGGCGT1001.8189894E-1229.24085631
GAGACCG1001.8189894E-1229.24085627
AGACCGG1001.8189894E-1229.24085628
GCGTAAA1250.026.9915634
CGTAAAT1350.024.99218635
AATCTCG1400.024.09960739
Produced by FastQC (version 0.11.5)