FastQCFastQC Report
Mon 6 Jun 2016
ERR1390623.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390623.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212698
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAACG300.00515447729.99153538
CTCGTAT709.3345443E-725.70596942
GATCGGA1052.3737812E-923.56574818
ATCGGAA1152.6557245E-1023.47163619
AGATCGG1104.0636223E-922.49457717
TATGCCG500.002268941822.49272243
TCGGAAG1201.1094016E-820.6183320
GGTACCG550.003942159520.44961514
ACGCCCG1009.0803223E-720.24428612
TCTCGTA706.9989933E-419.27947641
CGCCCGG1257.644332E-616.19609613
CCTTCGT1703.3287506E-815.9119881
CCGAGAC1451.8150295E-615.51222245
CGGCTAA1754.8781658E-815.42485317
ACCACGC2202.1827873E-1015.3359489
GCGGTTC1806.892515E-814.99638628
CGTTGGA2456.730261E-1114.7212955
GCCCGGC2152.590241E-914.64763314
CACGCCC2001.5801561E-814.6202711
ACGCCCA950.00538631414.20651612
Produced by FastQC (version 0.11.5)