FastQCFastQC Report
Mon 6 Jun 2016
ERR1390618.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390618.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191482
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTT250.002097284436.0190282
TCGTTGG450.00121905925.0132124
AGTTAGA450.001222780925.00014534
TCGTATA551.3647582E-424.5391869
CGTTGGA500.002250846722.5118925
CGTATAA500.002261127622.49425510
CTTCGTA550.003930559420.4493227
TTCGTAT706.9663784E-419.280798
TTCGTTG600.00647136518.7599093
TGTGATC600.00649093118.75010936
AAGTAGC600.006500731718.7452136
ACTAGAC901.855365E-417.50010119
GAGCCAC1108.508233E-414.3257494
AATCTCC1000.007533576313.4965527
ATATCTT1607.717935E-512.662943
TGAGCCA1701.3595552E-411.918063
AACTAGA1350.003986089511.66673518
TAGACAG1350.00399388311.66368921
TAGCTGG1350.00399388311.6636899
GAAATCT1400.005208435511.2559465
Produced by FastQC (version 0.11.5)