FastQCFastQC Report
Mon 6 Jun 2016
ERR1390615.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390615.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199578
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCCG301.1372525E-437.49603331
AGATCGA250.002085980736.0594371
ATCTTCG458.60844E-735.022625
TTCGTAT401.555263E-533.746438
TCGTATA352.813832E-432.1394589
TCGAGAC602.3884786E-730.019394
CTTCGTA554.1457206E-628.6333357
GTTCGAG406.12495E-428.1431792
ACTAGAC755.595939E-826.99714519
TCTTCGT608.153995E-626.2472236
AGTTCGA450.00121129225.0412771
TTCGAGA450.001218403225.016163
CGAGACC651.5079946E-524.2464335
TCTCGAA500.002259495222.49762210
ACCCCGT500.002259495222.49762232
ACCATCC602.459371E-422.497629
CCCCGTC550.00392772820.45238333
CGGCTCA550.00392772820.45238323
GTGAAAC951.3081464E-518.94536427
GAGACTA600.006496061618.74801613
Produced by FastQC (version 0.11.5)