FastQCFastQC Report
Mon 6 Jun 2016
ERR1390613.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390613.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221715
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG253.8818544E-544.9975229
AGACCGC253.8818544E-544.9975228
CCGAGAC356.2278577E-638.569325
CGTATGC301.1373214E-437.49793244
ACCGCGT301.1373214E-437.49793230
GAGACCG301.1373214E-437.49793227
CCGCGTA301.1373214E-437.49793231
CGCGTAG301.1373214E-437.49793232
CTCGTAT352.8139967E-432.14108742
CGTAGAA352.8139967E-432.14108734
TCTCGTA352.8139967E-432.14108741
TCGTATG352.8139967E-432.14108743
GCAGCCG300.005139791429.99834643
GCGTAGA406.152312E-428.12345133
ATCTCGT450.001223826824.99862140
AATGCCG450.001223826824.99862121
AAGAGCG500.002256655122.5038364
GAGCGGT500.00225961622.498766
ATGCCGA500.00225961622.4987622
CGGAAGA654.180114E-420.8009241
Produced by FastQC (version 0.11.5)