FastQCFastQC Report
Mon 6 Jun 2016
ERR1390612.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390612.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318818
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG250.002105730835.9968618
ATCGGAA250.002105730835.9968619
GATCGGA300.005143936329.99738718
AGCGCTC300.005143936329.9973877
GCGGTTC507.1466966E-526.99764628
CGCTCCA507.1466966E-526.9976469
ATAGGCT551.3659836E-424.54331629
AGCGGTT706.9724175E-419.28403527
GTTCAGC851.2017821E-418.52779631
CGGTTCA750.001108480617.99843229
ATGCCGA1004.1580308E-415.74862711
GTCCTGC900.003748172214.99869325
GACCTTT900.003748172214.99869322
CCGAGAT1150.001199233813.69445814
ACTAGAC1656.8781683E-613.63517619
AATGCCG1000.007539648613.49882310
CGAGATC1000.007539648613.49882315
CAGAAAC3450.013.04234237
TCTCAGA3803.6379788E-1211.84107434
GTTGGCC1350.00399883811.66564913
Produced by FastQC (version 0.11.5)