FastQCFastQC Report
Mon 6 Jun 2016
ERR1390610.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390610.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225818
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT450.001224171124.99766233
AGGTTGC908.209774E-619.99812936
GGTTGCA951.30925455E-518.94559737
TTGCAGT1153.426947E-617.6070539
GTTGCAG1153.426947E-617.6070538
AAGCATT2200.017.38473928
GAAGCAT2159.094947E-1216.7426227
GCATTCT1904.4201443E-1016.57739630
TAATCTT1104.6147274E-516.36210819
AGCATTC2101.05501385E-1016.06992529
AGAAGCA2304.1654857E-1014.67254226
TATCTTC2407.8216544E-1014.0705334
ACTAGAC1000.00753101213.49873819
GAGAATC1504.244558E-513.49873810
GAGCCAC1200.001642160913.1324984
GGAGGTT1200.001650404313.12377434
CACTTGA1250.002243411712.59882216
ATATCTT2951.690023E-811.4472123
CTGGTCT1450.006722625310.8779141
CAGAAGC2301.7185592E-510.75986425
Produced by FastQC (version 0.11.5)