FastQCFastQC Report
Mon 6 Jun 2016
ERR1390608.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390608.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113434
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA250.002101348735.9922410
GTCCATC250.002101348735.9922424
CGTTTCT300.005089198730.0465263
TTCGTAT300.005133352229.9935328
TCGTATA300.005133352229.9935329
AACTAGA608.120371E-626.2443418
ACTAGAC651.5105961E-524.22554619
AGCATTC903.239893E-722.49515229
TAGACAG908.159299E-619.9956921
AAACTAG807.4989584E-519.68325617
AAAACTA1051.4251837E-619.28155716
GCATTCT1002.0240856E-517.9961230
ATATCTT1153.3436354E-617.6360033
AATATCT1358.2805855E-716.6925132
AAGCATT1104.587E-516.3601128
ATTCTCA1257.5207754E-616.19650832
TATCTTC1301.073038E-515.601084
TTCTCAG1301.091179E-515.57356533
CTAGACA1209.647142E-514.99676620
GAAGCAT1209.647142E-514.99676627
Produced by FastQC (version 0.11.5)