FastQCFastQC Report
Mon 6 Jun 2016
ERR1390607.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390607.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137970
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT250.002101864435.995946
ACTAGAC802.9462826E-622.49746319
TAGACAG1109.837095E-820.45223821
AAGGTTA550.003921832420.45223841
AATATCC851.19242985E-418.534043
TATCTTC1354.179674E-818.3379124
GCAGATT750.001104340717.9979714
CTAGACA1305.698894E-717.3057420
TGCACTC850.00255177215.88056315
CTCAGAA1806.731352E-814.99830835
AATATCT1951.776225E-713.8546352
ATATCTT1951.7841194E-713.8496123
CAGATTC1000.007512461413.49847715
GCACTCC1000.007512461413.49847716
ATTCTCA2257.208109E-812.99853432
TCAGAAA2104.4167064E-712.85569436
AACTGCT1250.00223608212.59857841
TTCTCAG2207.762137E-712.27134333
GAAACTT1506.3918706E-411.99864739
CAAATAT1350.003883857311.7035291
Produced by FastQC (version 0.11.5)