FastQCFastQC Report
Mon 6 Jun 2016
ERR1390604.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390604.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315299
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACGC450.001224958324.9986769
CCACGCC551.3656187E-424.54415710
CACCACG654.230346E-420.7681338
CTTCGTA851.2014123E-418.5284337
ACTAGAC1601.6014383E-816.87410719
GAAGCAT2101.05501385E-1016.0705827
CTAGACA2403.6379788E-1215.93665720
ATCTTCG1004.1467376E-415.7541645
TCTTCGT1004.1567633E-415.7491676
TATCTTC3100.015.2459664
ATATCTT3650.014.7984333
CCACCAC2456.91216E-1114.6931017
AATATCT3850.014.6142532
AAGCATT2602.6320777E-912.98008328
AGCATTC2451.6008926E-812.85646329
GCCACCA2104.4744957E-712.8564636
TAGACAG3001.2551027E-1012.74932621
CAGAAGC2857.6215656E-1012.63091125
AAATATC5900.012.5980671
CAGAAAC3405.456968E-1212.57286537
Produced by FastQC (version 0.11.5)