FastQCFastQC Report
Mon 6 Jun 2016
ERR1390601.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390601.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241009
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA551.3605405E-424.5541735
GGACTAC754.5707442E-521.007463
ACTAGAC1301.2132659E-920.76798419
GCCCGGC654.226563E-420.76798414
TTCGTTG550.003919581420.461813
TCGTATA550.00392901920.453329
ATCTTCG706.9449475E-419.2925645
GGGACTA600.00647484418.7605532
TCGTTGG600.006482611418.7566594
TAGCATG600.00649816918.74887734
AAACGGG600.00649816918.74887711
TAGACAG1904.4201443E-1016.57795321
AACTAGA1652.3330358E-816.36265618
ATATCTT2701.8189894E-1215.0053283
TATCTTC2850.015.0053284
TCTTCGT900.0037439614.9991026
CTACAGG900.0037439614.9991026
AAACTAG1705.835882E-714.5579517
TTAGTAG1402.2167984E-514.46341935
AGTAGAG1251.374001E-414.399137537
Produced by FastQC (version 0.11.5)