FastQCFastQC Report
Mon 6 Jun 2016
ERR1390596.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390596.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216160
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTT500.002254123322.5078682
ACTAGAC903.264995E-722.49745619
CCTAGCT550.003928936620.45223215
GAAACTT1104.6140332E-516.36178639
TATCTTC1401.2386754E-616.077054
TAGACAG1552.0460175E-715.96593621
CTAGACA1654.1594467E-714.99830420
AAACTAG1250.00224320512.59857717
AAATATC2351.2578312E-712.4655871
ATATCTT2003.6092897E-612.3793273
AACTAGA1506.4178195E-411.99864318
GACAGAA2301.3395911E-611.73780323
CTGAGGC1953.7516707E-511.5558681
GCTGGTC1400.00523713711.24872822
AGACAGA2452.8641662E-611.01916222
GGCTGGT1650.001425884910.90785821
GAACTCC1450.00680004510.8608431
TCACTTG1450.00680004510.8608415
AATATCT2006.141361E-410.128542
ACAGAAG2302.0354084E-49.78150324
Produced by FastQC (version 0.11.5)