FastQCFastQC Report
Mon 6 Jun 2016
ERR1379404.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379404.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806071
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAACAATCTCGTATGCC13830.17157297558155546Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT2050.042.80446629
CCGATAA2100.041.78531331
CGATAAC2100.041.78531332
ACCGATA2200.039.8859830
CGTATGC2300.039.13005444
AGACCGA2300.039.13005428
CTCGTAT2250.037.99963442
TCGTATG2350.037.3400743
TCTCGTA2300.037.1735541
TGTTGCG250.002106835835.99965344
ATCTCGT2400.035.62465740
AGCGGTT2550.035.2937777
GCGGTTC2700.034.1663368
GAGACCG2700.034.16633627
GAGCGGT2700.033.3350756
GTATGCC2700.033.3330145
CGGTTCA2750.032.7269559
CCGAGAC2750.032.72695525
AGAGCGG2800.032.144545
ATGCCGA2950.030.5081822
Produced by FastQC (version 0.11.5)