FastQCFastQC Report
Mon 6 Jun 2016
ERR1379403.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379403.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651431
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAATTAGATCTCGTATGCC13940.2139904302988344Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAA1800.041.24929429
CTCGTAT1750.041.14215542
ATCTCGT1850.040.1344540
CGAATTA1850.040.1344532
ACCGAAT1950.039.23010330
GAGACCG1900.039.07827827
TCTCGTA1850.038.9182541
AGACCGA1950.038.07627528
CGTATGC1900.037.8940944
TCGTATG1950.036.9224543
CCGAATT2100.036.4279531
GTATGCC2200.033.74942445
CGGTTCA2400.032.811949
CGAGACC2350.032.5526426
GCGGTTC2450.032.1423078
AGAGCGG2500.031.5043015
AGCGGTT2450.031.2239577
AATGCCG2450.031.22395721
CCGAGAC2400.030.93697425
AAGAGCG2500.030.6041774
Produced by FastQC (version 0.11.5)