FastQCFastQC Report
Mon 6 Jun 2016
ERR1379402.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379402.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516982
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGTATATCTCGTATGCC20810.13718026977248246Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3000.038.24977544
CGAAGTA3050.037.6214932
CTCGTAT3050.036.8874642
ACCGAAG3150.036.4271630
TCGTATG3100.036.29010843
GACCGAA3100.036.28891429
AGCGGTT3300.035.453177
AGACCGA3200.035.1548928
TCTCGTA3400.034.41383441
GAGCGGT3400.034.4126976
GAGACCG3500.032.78444327
TATCTCG3550.032.32588639
CCGAAGT3550.032.3226931
GCGGTTC3700.031.6203968
CCGAGAC3800.031.3803625
AGAGCGG3750.031.2029025
ATCTCGT3700.031.01537740
ATGCCGA3800.030.78827922
AATGCCG3850.030.3884321
TCCGACT300.005142113730.0067481
Produced by FastQC (version 0.11.5)