FastQCFastQC Report
Mon 6 Jun 2016
ERR1379401.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379401.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673081
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA1950.040.38332732
ACCGAAC2150.037.67321830
GCGGTTC2200.036.817018
GACCGAA2150.036.6267429
CGTATGC2200.035.7943144
CTCGTAT2300.034.23803742
TCTCGTA2300.034.23803741
TCGTATG2350.033.5095743
GAGCGGT2450.033.0651136
CGGTTCA2550.032.6460159
AGACCGA2500.031.49899528
AGCGGTT2600.031.1528557
GAGACCG2600.031.15285527
CCGAGAC2550.030.88136525
ATCTCGT2800.029.73119440
CCGAACT2650.029.71603431
ATGCCGA2650.029.71603422
TGCCGAG2750.029.45360623
AAGAGCG2850.028.4269454
AATGCCG2850.028.42014721
Produced by FastQC (version 0.11.5)